TU Delft develops smart, energy-efficient purple bacteria cultivation systems. In Purple4Life, the team pioneers discontinuous lighting strategies and metabolic flexibility to reduce costs and environmental impact, making sustainable protein production economically viable.
Delft University of Technology: Innovation in Water-Energy-Biotechnology Systems
Delft University of Technology (TU Delft) is a world-leading technical university ranked 3rd globally in Water Resources. As a key partner in Purple4Life, TU Delft brings expertise in environmental biotechnology, water management, and sustainability assessment to develop cost-effective and circular PPB production systems.
Track record
TU Delft has pioneered PPB research through major European projects including NextGen (cultivating purple bacteria on brewery effluent), Saraswati 2.0 (pilot-scale PPB recovery from municipal sewage in India), and MELISSA (testing bacterial resilience in space missions). The team has developed advanced mechanistic models and control systems specifically for PPB cultivation, enabling smart and responsive bioprocess management.
Role in Purple4Life
TU Delft contributes to Work Package 1 on bioprocess optimization. Led by Dr. Ralph Lindeboom, the team develops an innovative cultivation strategy using fruit-based beverage process water and syngas, demonstrating metabolic flexibility that valorizes diverse industrial waste streams. TU Delft leads Task 1.4 on discontinuous lighting strategies—a breakthrough approach that reduces energy costs by coordinating PPB cultivation with real-time electricity prices and renewable energy availability.
Facilities and capabilities
- Automated photobioreactors (3L to 7L) with liquid and gas-fed configurations, plus open raceway reactors (100L) for pilot-scale studies.
- Comprehensive analytical suite including HPLC, LC-MS, GC-MS and UV-VIS for biomass characterization.
- Advanced bioinformatics capabilities with next-generation sequencing (Illumina MiSeq, Oxford Nanopore) and high-performance computational analysis.
- Mechanistic PBM models and model predictive control (MPC) systems for optimizing PPB production.
- Impact assessment tools integrating life cycle analysis with process design.
Networks and programs
TU Delft’s expertise extends through PurpleGain COST Action (Ralph organized the 3rd PurpleGain Workshop in Delft, 2023), PurpleDaze (interdisciplinary TU Delft initiative on PPB research), and international collaborations on resource recovery and circular water systems including EU H2020 Water-Mining and TRANSCEND projects on sustainable aviation fuels.
The team
Dr. Ralph Lindeboom (Assistant Professor, Water Management) leads process engineering and control systems development. Dr. Samarpita Roy (Assistant Professor, Biotechnology) contributes microbial ecology and metagenomics expertise to optimize bacterial metabolism and community function. Dr. Mar Palmeros Parada (Assistant Professor, Water Management) ensures sustainability and circularity through responsible innovation frameworks and life cycle assessments. Together, they bridge engineering, life sciences, and social sciences to create PPB production systems that work in the real world—economically, environmentally, and socially.
By Ralph Lindeboom (TU Delft)